SIBLINGS (BROTHER OR SISTER) UMBILICAL CORD BLOOD STEM CELLS TRANSPLANTATION FOR AUTISM TREATMENT
At the Mardaleishvili Medical Center sibling’s umbilical cord blood stem cells transplantation to patients with autism clinical study is conducted. Patients with childhood autism and autistic spectrum disorders can participate in the treatment. The treatment by nature is injection of patient’s sibling’s umbilical cord blood stem cells intravenously.
Patient is admitted to our clinic in the morning. On the empty stomach the patient has pre-surgery examinations done (complete blood count, coagulogram, immune phenotyping of lymphocytosis (CD3, CD4, CD8, CD19, CD25, CD56), determination of blood group and rhesus, serum screening for viruses (HIV, HCV, HBV), liver and kidney function (ALT, AST, total bilirubin, creatinine, blood urea, blood glucose), leukocytolysis – allergy tests to the products to be used for anesthesia of the patient, ECG, spinal X-ray, if necessary). If patient doesn’t have the results of any tests, which they had to admit before arriving to clinic (e.g. genetical test, or something else) these tests can be held here.
Next day, starting from morning on the empty stomach (minimum 5 hours before procedure the patient must not take food or drink) will be held the procedure of transplantation.
Defrosting of frozen (cryopreserved) umbilical cord blood cells and their preparation for transplantation
When matching HLA of patient and donor (brother or sister) minimum 50%, a tube or cryobag with donor’s frozen umbilical cord blood is placed into a water bath with the temperature of 37 °C. After 5 minutes the suspension will melt dawn and washing of cells with a buffer solution (DPBS) will be conducted, added substances will be completely removed from the suspension. The process is conducted at the laboratory and takes 30-40 minutes. Suspend the washed cells in 1 ml of physiological solution and send to the surgery. As soon as the cell suspension gets into the surgery unit, is conducted intravenous injection of Dormicum or Diazepam antianxiety drug to the patient. A specialist injects the prepared suspension intravenously.
In case of absence of complaints in the morning of day 2 the patient is discharged from the hospital.
The procedure is done in the surgery unit. Stem cells suspension is diluted in a 50 ml syringe infusion pump, diluted in 50 ml of physiological solution, and the transfusion goes on using the infusion pump for 10 minutes.
Monitoring of patient is conducted in the intensive care unit and in the post-surgery department. After awakening of the patient the monitoring continues in the post-surgery unit, and after 2-3 hours he (she) is transferred to a private ward, he (she) continues to be monitored for the detection of possible complications. In case of absence of complaints in the morning of day 2 the patient is discharged from the hospital.
About possible complications of using own umbilical cord blood stem cells
In case of use of own (autogenic) not multiplied umbilical cord blood stem cells, no hazard for the immune compatibility or any even insignificant theoretical genetic variation. According to the up to date experience no any serious omplications, including the risk of tumor development, have been reported in the use of many thousands of stem cells. In the complications include side effects, developed during the first days after the procedure. They are reported quite rarely and may be both side effects related to the injection of cells, and related to the anesthesia.
Early complications directly related to the administration of the cell suspension include:
- In rare cases mild hypersensitivity reactions may develop, for example, non-intensive allergic cough and erythema – hyperemia. Acute anaphylaxis is not described, theoretically it can’t be excluded, though the method used in preparation of stem cells almost completely excludes the injection of foreign allergens. As nerves system disorders patients may have mild excitability, which spontaneously resolves 1-2 days after the transplantation.
- The development of a severe hypersensitivity reaction may be expected only within the first hour after the transplantation. During this period the patient is monitored in the surgery unit.
For more detailed information, please contact to the International Department of clinic!